Specificity of bacteriolytic enzyme II from a soil amoeba, Hartmannella glebae
نویسندگان
چکیده
منابع مشابه
Growth and bacteriolytic activity of a soil amoeba, Hartmannella glebae.
A soil amoeba, Hartmannella glebae, could grow on a variety of gram-positive and gram-negative bacteria, although the rate of growth was faster in the presence of gram-negative bacteria. The amoeba, however, could not use yeasts, molds, or a green alga as a nutritional source. The extract prepared from amoebae grown in the presence of Aerobacter aerogenes and Alcaligenes faecalis could lyse int...
متن کاملPurification and specificity of bacteriolytic enzyme I from Hartmannella glebae.
A soil amoeba (Hartmannella glebae), when grown in conjunction with Enterobacter aerogenes and Alcaligenes faecalis, produced two enzymes. Enzyme I was purified by gel filtration on Sephadex G-100 and chromatography on diethylaminoethyl-cellulose. It is a basic protein. The analysis of the enzymic digest of the cell walls of Micrococcus lysodeikticus after reduction and acid hydrolysis showed t...
متن کاملMitrecin A, an endolysin-like bacteriolytic enzyme from a newly isolated soil streptomycete
UNLABELLED An open reading frame with homology to known endolysin genes was identified in the genome of Streptomyces sp. strain 212, which is a newly isolated soil bacterium. The heterologously expressed gene product of this endolysin-like gene, called Mitrecin A, demonstrated bacteriolytic activity against several Gram-negative bacteria. The genome of the bacterial strain was sequenced to draf...
متن کامل[Enrichment and characterization of a bacteriolytic amoebic enzyme].
Extracts of cultures of a small free-living amoeba, which feeds mainly on gram-negative bacteria, are capable of lysing heat-killed gram-negative bacteria. The lytic activity was enriched 36-fold by means of precipitation with (NH4)2S04 and of chromatography on Sephadex G-75, followed by ultrafiltration. The activity is less resistant to heat than is lysozyme, and is optimal at 45 °C. The pH-op...
متن کاملIdentification of "Ryan Virus" as an amoeba of the genus Hartmannella.
A recent paper (Pereira, et al., 1966) reported the isolation in Britain of several strains of an antibiotic-resistant infective agent that caused unusual cytopathic effects when inoculated into cell cultures. The new agent was evidently recoverable from the respiratory tract in certain patients, mostly young children, with illnesses of varying severity but with fever and respiratory symptoms a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1979
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.38.3.373-378.1979